BOTANY B.Sc. Part III 2012-13

 

 

 

VBS Purvanchal University ,Jaunpur

BOTANY

B.Sc. Part III

Paper- I MICROBIOLOGY AND PLANT PATHOLOGY AND PLANT PROTECTION

Unit-I

1. Introduction and significance Microbiology.

2. Methods of isolation and culturing of microbes.

3. An elementary study of immunology and serology.

4. Classification and distribution of microorganism in nature.

Unit-II

1. Decomposition of organic matter is soil, Role of microorganism in the

cycle of carbon and nitrogen.

2. Industrial utilization of microorganisms. Elementary idea of microbiology

of milk, alcohol, antibiotics, vitamins and organic acids.

Unit-III Plant Pathology

1. History and scope of plant pathology.

2. General symptoms of fungal, bacterial and viral diseases.

3. Mode of infection, disease resistance and control.

Unit-IV

1. A study of following diseases with reference to symptoms, causal

organisms, diseases cycle and control. Late blight of Potato, Powdary

mildew of pea. Tikka disease of ground nut, yellow vein mosaic of bhindi,

Bacterial blight of rice, Red rot of sugar cave, Little leaf.

Practical

1. Isolation of microorganisms from natural resources.

2. Staining and measurement of microbial cells.

3. Study of host parasite relationship of plant diseases listed above.

PAPER-II ECOLOGY, ENVIRONMENTAL POLLUTION, CONSERVATION

AND FORESTRY:

Unit-I

1. Basis principles of ecology

2. Ecological factors: climate, edaphic, biotic and topographic factors.

3. Biotic communities and developments of vegetation, Halophytes and

Epiphytes.

Unit-II Ecosystem Ecology

1. Ecosystem with reference to grass land, forest and pond, idea of food

chain, pyramids of number and biomass.

2. Energy flow and productivity.

3. Biogeochemical cycles, C.N.P. and Hydrological cycle.

Unit-III Atmospheric Pollution

1. Brief idea of Lithosphere, hydrosphere and atmosphere.

2. Brief idea of biospheric pollution causes and effects, water pollution air

pollution, noise pollution.

3. Techniques and devices to control different types of pollutions.

Unit-IV Conservation

1. Conservation of soil and renewable resources.

2. Major forest types of India and their characteristic features.

3. Afforestation, Agro forestry ad social forestry in India.

Practical

Study of communities by quadrat method to workout frequency, density and

abundance.

Biomass estimation

Determination of physical and chemical characteristics of water and air.

Determination of sound levels at different places.

Study of acute symptoms in the plants growing along road sides.

PAPER-III PLANT ANATOMY & EMBRYOLOGY

Unit-I Plant Anatomy

1. Meristems.

2. Leaf anatomy.

3. Origin, structure and function of the vascular cambium including

anamolous behaviour.

4. Structure of Xylem and Phloem.

Unit-II

1. Cork cambium activity and its products.

2. Structure and characteristics of wood.

Unit-III Embryology

1. Structure of anther, microspogenesis and development of the male

gametophyte.

2. Structure of ovule, megasporogenesis and development of the female

gametophyte with particular reference to polygonum type.

Unit-IV

1. Fertilization, Endosperm and embryo development (onagrad type)

2. Apomixis, Polyembryony and Parthenocarpy.

PRACTICAL

1. Plant Anatomy, Anatomy of normal Dicot and Monocot roots and stems.

Anatomy of anamolous structure of stems of: Bignonia, Nyctanthes,

Achyranthes, Boerhaavia, Dracaena.

Paper-IV GENETIC ENGINEERING AND BIOTECHNIQUE

Unit-I

1. Elementary idea of gene synthesis.

2. Tools and techniques of recombinant DNA Technology, Vectors and

restriction enzymes.

3. General principles of gene cloning.

Unit-II

Characterization and analysis of clone, blotting techniques, PCR and it application

in DNA finger printing.

Application of genetic engineering to human welfare.

Transgenic plants and gene transfer technology in them.

Unit-III

Elementary idea of cell culture and its nutrient media, protoplast fusion and

hybridomas.

Practical application of tissues and organ culture.

Micropropogation : auxilary bud, short tip and meristem culture, factors affecting

the micropropogation.

Haploid production and their application.

Unit-IV

Spectrophotometry, Biosensor, Cryoelectron microscopy.

Application of X-ray crystellography and NMR (Biology) spectroscopy.

Separation techniques – chromatography.

Practicals

Preparation of various nutrient media for tissue culture.

Estimation of DNA, RNA from plant samples.

Demonstration of photographs of

DNA Structure and replication

Gene cloning

Cellus

Micropropagation

Demonstration of cellus formation from pith of carrot root.

Separation of cell constituents by using chromatography techniques.

Practical Examination Scheme Based on Paper I, II, III and IV

Microbiology experiment

Plant pathology (i) Host parasite relationship

Experiment on Ecology/Environmental Biology/Forestry.

Temporary mounting of a double stained section of material for anatomical

studies. Identification of material with suitable comments and sketch.

Embryo dissection.

Experiments on Genetic Engineering and instrumentation.

Identify and comment upon spots (1 to 8).

Viva-Voce.

Records

Collection/Model/Charts.